Pancreatic elastasa in Calais Invitro. What is elastas kala. Preparation for analysis and collection of biological material

01.08.2019 Information

Pancreatic elastase is present in the body of every person literally from his very birth. The deficiency of this enzyme in the body can lead to diseases of the gastrointestinal tract, pancreas, the development of pancreatitis.

The role of elastase in the human body

Elastasa (coprological elastase, pancreatteptidase E) is one of the few enzymes in the human body, which does not change when passing through the intestine. Therefore, by conducting a feast analysis on pancreatic elastasia, it is possible to estimate the current state of the pancreas, the main function of which the synthesis of most enzymes entering the intestines along with pancreatic juice.

What other names for this test?

This test measures the amount of elastase 1 in the sample of the chair. This test is used to diagnose certain diseases of the pancreas associated with the insufficient number of digestive enzymes. Laboratory tests can be carried out for many reasons. Tests are conducted for routine health screening or if disease or toxicity is suspected. Lab tests can be used to determine whether health is improved or deteriorates. Laboratory tests can also be used to measure the success or failure of drugs or treatment plan.

The task of elastase is to split protein, elastics and other proteins that a person gets along with food.

Elastin has, in essence, only one analogue is collagen. However, it contains collagen exclusively in food of animal origin, while elastin is in animals and vegetable products. Regular use of foods containing this enzyme, well affect the skin, hair, the work of the gastrointestinal tract.

Laboratory tests can be ordered by professional or legal reasons. This test may be required if you have. When and how often laboratory tests are conducted, it may depend on many factors. The time of laboratory tests can be based on the results or completion of other tests, procedures or treatment. Lab tests can be performed immediately in emergency, or tests can be postponed when the state is processed or controlled.

The test may be offered or become necessary when certain signs or symptoms appear. Because of the changes in how your body is naturally functioning during the day, laboratory tests may be required at a certain time of the day. If you have prepared a test by changing the consumption of food or liquid, laboratory tests can be calculated in accordance with these changes. Test time can be based on increasing and decreasing the number of drugs, medicines or other substances in the body.

Elastas in Kale will be present in children and adults anyway. The question is whether the person has enough enzyme produced by the body or less norms. With an increased or reduced value, you should consult with your doctor and take appropriate actions.

In what cases is the analysis

The age or sex of the inspected person may affect the time and time when the laboratory test is required. Chronic or progressive conditions may require constant monitoring using laboratory tests. Conditions that worsen and improving can also need a frequent observation. Some tests can be repeated to obtain a number of results, or tests may be needed to be repeated to confirm or refute the results. Dates and frequency of laboratory tests may differ if they are performed on professional or legal reasons.

You can hand over the analysis on pancreatic elastasia in any diagnostic center, since this study is included in the list of standard analyzes.

How to pass the analysis?

Callery masses of a person before surrender does not require special training. It consists in collecting samples and their surrender. The direction for the analysis of the Elastase enzyme can issue a surgeon or a gastroenterologist after consulting and the appropriate examination.

How do I prepare for the test?

Before giving a sample chair, inform the health care worker if you have diarrhea or you use antibiotics, barium, bismuth, oil, iron, magnesium or medication to stop diarrhea. For sample chair, you will be asked to translate the intestine to a special container. Avoid adding urine, water, fabrics or toilet paper to the sample chair.

The number of discomfort you feel will depend on many factors, including from your sensitivity to pain. Tell us how you feel with a person who performs the test. Tell a person performing the test if you feel that you cannot continue the test.

The signal to the fact that the analysis should be given, the following symptoms may be:

painful unpleasant and bright sensations in the abdomen;
concentrated pain;
cal acquires a yellowish shade;
eye proteins and skin yellow;
there are suspicions after the ultrasound (inflammation of the pancreas).

The chair must be handed over to a special study in a diagnostic laboratory in a sterile container that you can purchase in any pharmacy. Almost nothing affects the distortion of the results, because to stop receiving medicinal preparations Or somehow change the diet is not necessary.

What should I do after the test?

This test usually does not cause discomfort. After passing the sample of the chair in medical institution, Close the container if it has a lid, and place the container in which the health instructor instructed. Clean your hands with water with soap. If you were asked to build a sample of a chair at home, follow the instructions.

What are the normal results for this test?

Stool: Giving a sample of stool is usually considered safe. Talk to your healthcare employee if you have questions or concerns about this test. The results of laboratory tests may vary depending on your age, gender, disease history, method used for test, and many other factors. If your results differ from the results proposed below, it may not mean that you have a disease. If you have any questions, contact your health care person.

The standard container in which the feces analysis is rented is recommended to fill in about 30-40%. If the collection of a child or an adult was held in the evening, then the container should be placed on the night in the refrigerator and stored at temperatures in the range of 2-6 degrees. This will allow the elastas to preserve and achieve optimal research results.

What can affect the results of my tests?

For this test is considered a normal result. Results decreased in the non -naretive diarrhea due to changes in the composition of the chair or water content.

What should be done after this test

Ask your medical worker as you will be informed about the test results. You may be asked to cause results, appoint a meeting to discuss the results or report results by mail. Subsequent help depends on many factors associated with your test. Sometimes after you have been notified of the test results, no follow-up occurs.

The only exception is the use of laxatives or rectal candles To give the samples. They negatively affect the accuracy of the result.

Serum elastaza

In suspects of disorders of the GTS and the pancreas, the child or adult, the analysis can be made on the basis of feces or blood serum. The study of serum elastase is prescribed in the case of abdominal pain in the patient.

In other cases, it may be proposed or necessary to take action. Some examples of subsequent care include changes in the plans of treatment or treatment, the direction of a specialist, more or less frequent observation and additional tests or procedures. Talk to your healthcare employee about any problems or questions that you have relatively follow-up actions or instructions.

In an attempt to resolve or eliminate the presence of a single disease or the absence of another, clinicians often turn to clinically approved stool-based tests that measure different biomarkers. The measurement of pancreatic elastase to diagnose the disease of the pancreas has become more common practice of clinicians. The levels of pancreatic elastase chairs can help determine the exocrine pancreatic function. This is a tool that allows you to confidently measure the levels of pancreatic elastase in the samples of the human chair.

At the same time it happens two types:

Pancreatic elastase 1. This is an enzyme, the molecular weight of which is 30,000. The substance is produced by the pancreas and circulates inside the body free blood. Or can circulate with an inhibitor that suppresses the activity of the enzyme.
Pancreatic elastasa 2. Its molecular weight is 25,000. Make the analysis is recommended if there is suspicion of jade, arthritis or emphysee.

Together with blood surveys and feces on Elastasia, doctors can assist the analysis of another enzyme - pancreatic lipase. This is a pancreas enzyme, whose task is to split fatty acids, fats and glycerol.

Measurement of pancreatic elastase for diagnosis of pancreatic disease

The elastase of the pancreas is mainly associated with the bile salts during the passage of the intestine and does not degrade 1. As a result, the levels of pancreatic elastase in the person's chair 5-6 times more concentrated than in the pancreas 1. The concentration of the chair reflects the secretory ability of the pancreas, the pancreas tablet reflects Elastase is a marker used to determine the exocrine function of the pancreas, with low levels indicating the presence of pancreatic insufficiency, which can be the result of a number of different diseases, such as chronic pancreatitis, pancreatic cancer, cystic fibrosis, diabetes and Crohn's disease.

What do the results say?

Increases the level of elastase. Incorrect meals, lack of useful products in the diet, as well as related diseases of the gastrointestinal tract and pancreas.

After you surrender samples, they will be directed to an enzyme immunoassay study. The doctor, having received the results, can say lower or higher than the level of the enzyme in your feces. With this diagnosis, serious pathological diseases are detected. This makes it possible in a timely manner. early stages Their development, proceed to treatment.

These factors contributed to the growth of measuring pancreatic elastase for the diagnosis of pancreatic disease. It is extremely important to trust your results when testing the levels of pancreatic elastase in a chair. Possessing the widest range and most sensitivity, less often repeats less samples. The system of sandwich monoclonal antibodies provides increased specificity, which provides greater confidence in measuring the pancreatic elastase to diagnose the pancreas.

An analysis is compatible with our unique, which saves the time and money of researchers in the procedures for extraction of the chair. Conclusion. Evaluation of the fecal elastase 1 is not much superior to the traditional assessment of fecal chymotrypsin. Fecal elastasa 1Pecal chimotrypsinscretin-panopozimine test infracks Announced pancreas insufficiency diagnosis. The diagnosis of chronic pancreatitis is usually based on the anomalous results of the tests of the pancreas function and morphological research.

Decoding results is not complicated, because you can understand them yourself:

Norma for healthy man - It is at least 200 μg of the enzyme per unit of measurement. If the study showed below this mark, it will be necessary to pass additional diagnosis In order to identify the reasons that caused low elastasia. Low level Speaks about the ineffective work of the PJ.
The interval from 200 to 500 units is the optimal amount of enzyme for a healthy human pancreas.
Reduced elastase is a range of 101 to 199 units, indicates the middle or easier enzyme failure.
If, as a result of the survey, a level of up to 100 units was detected - the pancreas suffered greatly and there is a severe form of the disease.
Over 500 units of the enzyme in samples with a minor exceeding this plank does not say anything dangerous. But with the aim of prevention and calm, it is better to re-repeated or pass additional analyzes. In some cases, the error is possible.
Cala analysis can show 700-1000 units. In such situations there is a suspicion of oncological diseases, pancreatitis and bile disease. It is necessary to undergo a full-fledged examination in order to accurately detect the disease.

To estimate the exocrine function of the pancreas, both direct and indirect tests are carried out. The gold standard is a secret pancrozimine or one of its modifications. However, this can be performed only in qualified gastroenterological centers, since the test is a time-consuming, invasive and expensive. None of them became a recognized gold standard for doctors in clinical practice Out of gastroenterological centers. Therefore, interest is the discharge of pancreatic elastase 1 and its further characteristic of the enzyme specific for humans and pancreatic gland, which does not degrade during intestinal transport and which is enriched 5-6 times with feces compared to duodenal juice.

With a shortage of the production of pancreas juice, not only pancreatitis can be suspected, but also other aids:

pancreatic cancer;
hepatitis;
intestinal pathology;
mukobovysitosis;
lactose intolerance;
crown disease;
stones.

To determine the level of pancreatic elastase 1 in feces is recommended periodically to each of us, regardless of age or floor. It is very important when identifying violations to accurately determine with which the deviations from the norm in the enzyme indicators are associated.

A highly sensitive immunoassay analysis with enzyme binding for fecal and duodenal elastase 1 of a person using two specific monoclonal antibodies is commercially available. Preliminary studies seem to indicate that the assessment of the fecal elastase 1 is a valuable test of the pancreas function and can become a new standard indirect test of the pancreas. In addition, we sought to find out whether this new test is really better than the traditionally used assessment of fecal chymotrypsin.

Only the doctor gives the appropriate directions for analysis and further diagnostics. Do not attempt to engage in self-medication. After the first body signal, go to the clinic. Even if the work of the pancreas does not bother you, try to give a complex of analyzes once a year. This applies not only to elastase, but also urine studies, blood. Such a relation to your own health is absolutely correct. In addition, the tests are quite accessible and carried out in any clinic.

The last three tests were carried out on three samples of the chair collected in 24 hours for three days, and average values \u200b\u200bwere identified. Thus, the specificity of the determination of elastase 1 was 94%, and for chymotrypsin 91 and 79% - depending on the upper limit of normality. In a severe group, the percentage of abnormal test results was high, 82% of the abnormal results for elastase 1 and 73 and 91% to evaluate chymotrypsin for the two lower limits of the norm.

Evaluation of the fecal elastase 1 and fecal chymotrypsin in patients with exocrine non-sufficiency of the pancreas various degrees severity. Evaluation of the fecal elastase 1 and fecal chymotrypsin in patients with compensated and decompensated exocrine non-sufficiency of the pancreas.

Diseases of the digestion system are dangerous in that they are able to develop very quickly and move into heavy forms, to cure that then much more difficult.

Pancreatic elastase 1-proteolytic enzyme of the digestive system, produced exclusively PJ. Elastasa splits the internal associations of protein (neutral amino acids). It is present in human pancreatic juice and feces. The enzyme is not exposed to passing intestinal tract. The concentration of pancreatic elastase 1 in feces is 5-6 times higher than in pancreatic juice. High stability of pancreatic elastase 1 allows not to limit the time of delivery of the analysis to the diagnostic laboratory. Material for defining E1 can be stored during the week in the refrigerator. One feces sample is enough to form a diagnosis (there is no need to assemble the daily feces). Definition E1 is used to evaluate the exocrine function of the pancreas. In contrast to fecal chymotrypsin, the results of the definition of E1 do not depend on the administration of pancreatic enzymes by patients.

Evaluation of the fecal elastase 1 and fecal chymotrypsin in patients with exocrine pancreatic deficiency and morphological changes in the pancreas pointing to chronic pancreatitis. They significantly differ in the diagnostic criteria of chronic pancreatitis, diagnostic gold, with which the assessment of the fecal enzyme, the severity of the pancreas function or chronic pancreatitis and, is equally important, the number of patients studied. Specificity was 83% for estimating elastase 1 and 92% to evaluate chymotrypsin.

Replacement therapy increases the activity of chymotrypsin in feces and does not have actions on elastasia. Focusing on the level of elastase 1 in feces, you can more accurately assign enzyme preparations And make the prognosis of the disease. With the development of the lack of exocrine pancreas function, the content of pancreatic E1 decreases in Kale. Reducing E1 activity in Kale is also detected in patients with chronic pancreatitis, pancreatic cancer, in children with cystic fibrosis. In case of fibergation, it is recommended to explore the level of elastase 1 in feces at least once every 6 months. The E1 content in feces does not change in a number of other diseases: celiac disease, inflammatory bowel diseases, infectious diarrhea. According to the results, it is possible not only about the level of enzyme deficiency of PJ, but also by conducting regular studies, evaluate the exocrine function in the dynamics. The E1 content at CP is reduced to a level of less than 150 μg / g. The specificity of the test during the construction of the feces is 93%, the sensitivity is 93%. The method is deprived of the deficiencies of the "Gold Standard" - secretine-Cerulino direct test: the lack of standardization, inaccurately defined lower boundary normal values, high discomfort for patients, analysis duration and high cost.

CJSC Biokhimmak offers an immunoopimen system of the company "Bioserv AG" for the diagnosis of exocrine deficiency of PJ.

In this method, two polyclonal antibodies used simultaneously are specifically identified by elastase 1. Synthetic protein was used to immunize animals, similar to human pancreatic elastase 1. Specific polyclonal antibodies recognize when analyzing approximately 50-70 epitopes of human pancreatic elastase 1, unlike the test system Other manufacturers that use only two monoclonal antibodies that recognize no more than two different epitopes. If these epitopes are hidden, which happens quite often, the test will give falsely understated / false positive results (low diagnostic specificity).